| Design of Ca2+‐independent Staphylococcus aureus sortase A mutants H Hirakawa, S Ishikawa, T Nagamune Biotechnology and bioengineering 109 (12), 2955-2961, 2012 | 105 | 2012 |
| Molecular assembly of P450 with ferredoxin and ferredoxin reductase by fusion to PCNA H Hirakawa, T Nagamune ChemBioChem 11 (11), 1517, 2010 | 99 | 2010 |
| Ca2+‐independent sortase‐A exhibits high selective protein ligation activity in the cytoplasm of Escherichia coli H Hirakawa, S Ishikawa, T Nagamune Biotechnology journal 10 (9), 1487-1492, 2015 | 87 | 2015 |
| Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site Y Yamamura, H Hirakawa, S Yamaguchi, T Nagamune Chemical communications 47 (16), 4742-4744, 2011 | 71 | 2011 |
| Intramolecular electron transfer in a cytochrome P450cam system with a site-specific branched structure H Hirakawa, N Kamiya, T Tanaka, T Nagamune Protein Engineering, Design & Selection 20 (9), 453-459, 2007 | 58 | 2007 |
| Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1 H Hirakawa, N Kamiya, Y Kawarabayashi, T Nagamune Journal of bioscience and bioengineering 97 (3), 202-206, 2004 | 51 | 2004 |
| Supramolecular protein assembly supports immobilization of a cytochrome P450 monooxygenase system as water-insoluble gel CY Tan, H Hirakawa, T Nagamune Scientific reports 5 (1), 8648, 2015 | 46 | 2015 |
| Fine tuning of spatial arrangement of enzymes in a PCNA-mediated multienzyme complex using a rigid poly-L-proline linker T Haga, H Hirakawa, T Nagamune PloS one 8 (9), e75114, 2013 | 45 | 2013 |
| Immobilization of a bacterial cytochrome P450 monooxygenase system on a solid support CY Tan, H Hirakawa, R Suzuki, T Haga, F Iwata, T Nagamune Angewandte Chemie International Edition 55 (48), 15002-15006, 2016 | 35 | 2016 |
| Log P effect of organic solvents on a thermophilic alcohol dehydrogenase H Hirakawa, N Kamiya, Y Kawarabayashi, T Nagamune Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1748 (1), 94-99, 2005 | 30 | 2005 |
| Introduction of selective intersubunit disulfide bonds into self‐assembly protein scaffold to enhance an artificial multienzyme complex's activity H Hirakawa, A Kakitani, T Nagamune Biotechnology and Bioengineering 110 (7), 1858-1864, 2013 | 28 | 2013 |
| Artificial protein complexes for biocatalysis H Hirakawa, T Haga, T Nagamune Topics in Catalysis 55, 1124-1137, 2012 | 26 | 2012 |
| Electron donation to an archaeal cytochrome P450 is enhanced by PCNA‐mediated selective complex formation with foreign redox proteins R Suzuki, H Hirakawa, T Nagamune Biotechnology journal 9 (12), 1573-1581, 2014 | 24 | 2014 |
| Regioselective reduction of a steroid in a reversed micellar system with enzymatic NADH-regeneration H Hirakawa, N Kamiya, T Yata, T Nagamune Biochemical engineering journal 16 (1), 35-40, 2003 | 21 | 2003 |
| Crystal structure of histamine dehydrogenase from Nocardioides simplex T Reed, GH Lushington, Y Xia, H Hirakawa, DAM Travis, M Mure, ... Journal of biological chemistry 285 (33), 25782-25791, 2010 | 20 | 2010 |
| Phosphite‐driven Self‐sufficient Cytochrome P450 H Watanabe, H Hirakawa, T Nagamune ChemCatChem 5 (12), 3835-3840, 2013 | 16 | 2013 |
| Site-selective and stepwise complexation of two M (cod)+(M= Rh, Ir) fragments with calix [4] arene Y Ishii, K Onaka, H Hirakawa, K Shiramizu Chemical communications, 1150-1151, 2002 | 15 | 2002 |
| Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A J Zhang, S Yamaguchi, H Hirakawa, T Nagamune Journal of bioscience and bioengineering 116 (3), 298-301, 2013 | 11 | 2013 |
| Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes H Hirakawa, T Nagamune Enzyme Engineering: Methods and Protocols, 149-163, 2013 | 6 | 2013 |
| Artificial Self‐Sufficient Cytochrome P450 Containing Multiple Auxiliary Proteins Demonstrates Improved Monooxygenase Activity T Haga, H Hirakawa, T Nagamune Biotechnology Journal 13 (12), 1800088, 2018 | 5 | 2018 |
