Kei Fujiwara
Kei Fujiwara
Dept. Biosciences and Informatics, Keio University
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A systematic survey of in vivo obligate chaperonin‐dependent substrates
K Fujiwara, Y Ishihama, K Nakahigashi, T Soga, H Taguchi
The EMBO journal 29 (9), 1552-1564, 2010
Cell-sized confinement in microspheres accelerates the reaction of gene expression
A Kato, M Yanagisawa, YT Sato, K Fujiwara, K Yoshikawa
Scientific reports 2, 283, 2012
DNA cytoskeleton for stabilizing artificial cells
C Kurokawa, K Fujiwara, M Morita, I Kawamata, Y Kawagishi, A Sakai, ...
Proceedings of the National Academy of Sciences 114 (28), 7228-7233, 2017
Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system
K Fujiwara, T Katayama, SM Nomura
Nucleic acids research 41 (14), 7176-7183, 2013
Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristic of individual F-BAR domain proteins
Y Tanaka-Takiguchi, T Itoh, K Tsujita, S Yamada, M Yanagisawa, ...
Langmuir, 2012
Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE
K Fujiwara, H Taguchi
Journal of bacteriology 189 (16), 5860-5866, 2007
Condensation of an additive-free cell extract to mimic the conditions of live cells
K Fujiwara, SM Nomura
PloS one 8 (1), e54155, 2013
Biochemical preparation of cell extract for cell-free protein synthesis without physical disruption
K Fujiwara, N Doi
PloS one 11 (4), e0154614, 2016
Generation of giant unilamellar liposomes containing biomacromolecules at physiological intracellular concentrations using hypertonic conditions
K Fujiwara, M Yanagisawa
ACS synthetic biology 3 (12), 870-874, 2014
Universal glass-forming behavior of in vitro and living cytoplasm
K Nishizawa, K Fujiwara, M Ikenaga, N Nakajo, M Yanagisawa, D Mizuno
Scientific reports 7 (1), 1-12, 2017
Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation
M Yanagisawa, S Nigorikawa, T Sakaue, K Fujiwara, M Tokita
Proceedings of the National Academy of Sciences 111 (45), 15894-15899, 2014
Droplet‐Shooting and Size‐Filtration (DSSF) Method for Synthesis of Cell‐Sized Liposomes with Controlled Lipid Compositions
M Morita, H Onoe, M Yanagisawa, H Ito, M Ichikawa, K Fujiwara, H Saito, ...
ChemBioChem 16 (14), 2029-2035, 2015
Introducing Micrometer-Sized Artificial Objects into Live Cells: A Method for Cell–Giant Unilamellar Vesicle Electrofusion
AC Saito, T Ogura, K Fujiwara, S Murata, SM Nomura
PloS one 9 (9), e106853, 2014
Human-derived fusogenic peptides for the intracellular delivery of proteins
K Sudo, K Niikura, K Iwaki, S Kohyama, K Fujiwara, N Doi
Journal of Controlled Release 255, 1-11, 2017
Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device
H Yamashita, M Morita, H Sugiura, K Fujiwara, H Onoe, M Takinoue
Journal of bioscience and bioengineering 119 (4), 492-495, 2015
Conversion of a chaperonin GroEL-independent protein into an obligate substrate
T Ishimoto, K Fujiwara, T Niwa, H Taguchi
Journal of Biological Chemistry 289 (46), 32073-32080, 2014
PURE mRNA display for in vitro selection of single-chain antibodies
Y Nagumo, K Fujiwara, K Horisawa, H Yanagawa, N Doi
The Journal of Biochemistry 159 (5), 519-526, 2016
Increasing elasticity through changes in the secondary structure of gelatin by gelation in a microsized lipid space
A Sakai, Y Murayama, K Fujiwara, T Fujisawa, S Sasaki, S Kidoaki, ...
ACS central science 4 (4), 477-483, 2018
In vitro transcription–translation using bacterial genome as a template to reconstitute intracellular profile
K Fujiwara, T Sawamura, T Niwa, T Deyama, SM Nomura, H Taguchi, ...
Nucleic Acids Research 45 (19), 11449–11458, 2017
Artificial cell fermentation as a platform for highly efficient cascade conversion
K Fujiwara, T Adachi, N Doi
ACS Synthetic Biology 7 (2), 363-370, 2018
論文 1–20