Reversal of indoleamine 2, 3-dioxygenase–mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme TA Triplett, KC Garrison, N Marshall, M Donkor, J Blazeck, C Lamb, ... Nature biotechnology 36 (8), 758-764, 2018 | 263 | 2018 |
Recent advances in controlled immobilization of proteins onto the surface of the solid substrate and its possible application to proteomics K Nakanishi, T Sakiyama, Y Kumada, K Imamura, H Imanaka Current Proteomics 5 (3), 161-175, 2008 | 135 | 2008 |
Screening and characterization of affinity peptide tags specific to polystyrene supports for the orientated immobilization of proteins Y Kumada, Y Tokunaga, H Imanaka, K Imamura, T Sakiyama, S Katoh, ... Biotechnology progress 22 (2), 401-405, 2006 | 101 | 2006 |
Preparation of monodisperse and size-controlled poly (ethylene glycol) hydrogel nanoparticles using liposome templates SY An, MPN Bui, YJ Nam, KN Han, CA Li, J Choo, EK Lee, S Katoh, ... Journal of colloid and interface science 331 (1), 98-103, 2009 | 74 | 2009 |
On the interaction site of serine acetyltransferase in the cysteine synthase complex from Escherichia coli C Zhao, Y Moriga, B Feng, Y Kumada, H Imanaka, K Imamura, ... Biochemical and biophysical research communications 341 (4), 911-916, 2006 | 66 | 2006 |
Effects of cytoplasmic and periplasmic chaperones on secretory production of single-chain Fv antibody in Escherichia coli H Sonoda, Y Kumada, T Katsuda, H Yamaji Journal of bioscience and bioengineering 111 (4), 465-470, 2011 | 64 | 2011 |
Development of a one-step ELISA method using an affinity peptide tag specific to a hydrophilic polystyrene surface Y Kumada, S Katoh, H Imanaka, K Imamura, K Nakanishi Journal of biotechnology 127 (2), 288-299, 2007 | 54 | 2007 |
The bialaphos resistance gene (bar) plays a role in both self-defense and bialaphos biosynthesis in Streptomyces hygroscopicus Y Kumada, H ANZAI, E Takano, T Murakami, O Hara, R Itoh, S IMAIT, ... The Journal of Antibiotics 41 (12), 1838-1845, 1988 | 53 | 1988 |
Site-specific immobilization of recombinant antibody fragments through material-binding peptides for the sensitive detection of antigens in enzyme immunoassays Y Kumada Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1844 (11), 1960-1969, 2014 | 50 | 2014 |
Characterization of polystyrene-binding peptides (PS-tags) for site-specific immobilization of proteins Y Kumada, D Kuroki, H Yasui, T Ohse, M Kishimoto Journal of bioscience and bioengineering 109 (6), 583-587, 2010 | 50 | 2010 |
Direct immobilization of functional single-chain variable fragment antibodies (scFvs) onto a polystyrene plate by genetic fusion of a polystyrene-binding peptide (PS-tag) Y Kumada, K Hamasaki, Y Shiritani, A Nakagawa, D Kuroki, T Ohse, ... Analytical and bioanalytical chemistry 395, 759-765, 2009 | 50 | 2009 |
Cloning, overexpression, purification, and characterization of O-acetylserine sulfhydrylase-B from Escherichia coli C Zhao, Y Kumada, H Imanaka, K Imamura, K Nakanishi Protein expression and purification 47 (2), 607-613, 2006 | 48 | 2006 |
Replacement of Streptomyces hygroscopicus genomic segments with in vitro altered DNA sequences H Anzai, Y Kumada, O Hara, T Murakami, R Itoh, E Takano, S IMA, ... The Journal of Antibiotics 41 (2), 226-233, 1988 | 47 | 1988 |
Functional expression of single-chain Fv antibody in the cytoplasm of Escherichia coli by thioredoxin fusion and co-expression of molecular chaperones H Sonoda, Y Kumada, T Katsuda, H Yamaji Protein expression and purification 70 (2), 248-253, 2010 | 46 | 2010 |
The cloned Streptomyces bikiniensis A-factor determinant S HORINOUCHI, M NISHIYAMA, H SUZUKI, Y KUMADA, T BEPPU The Journal of Antibiotics 38 (5), 636-641, 1985 | 40 | 1985 |
Protein–protein interaction analysis using an affinity peptide tag and hydrophilic polystyrene plate Y Kumada, C Zhao, R Ishimura, H Imanaka, K Imamura, K Nakanishi Journal of biotechnology 128 (2), 354-361, 2007 | 39 | 2007 |
High biological activity of a recombinant protein immobilized onto polystyrene Y Kumada, Y Shiritani, K Hamasaki, T Ohse, M Kishimoto Biotechnology Journal: Healthcare Nutrition Technology 4 (8), 1178-1189, 2009 | 37 | 2009 |
Production of single-chain variable fragment antibody (scFv) in fed-batch and continuous culture of Pichia pastoris by two different methanol feeding methods S Yamawaki, T Matsumoto, Y Ohnishi, Y Kumada, N Shiomi, T Katsuda, ... Journal of bioscience and bioengineering 104 (5), 403-407, 2007 | 37 | 2007 |
The bialaphos biosynthetic genes of Streptomyces viridochromogenes: cloning, heterospecific expression, and comparison with the genes of Streptomyces hygroscopicus O Hara, T Murakami, S Imai, H Anzai, R Itoh, Y Kumada, E Takano, ... Microbiology 137 (2), 351-359, 1991 | 37 | 1991 |
Production kinetics of angiotensin-I converting enzyme inhibitory peptides from bonito meat in artificial gastric juice F Hasan, M Kitagawa, Y Kumada, N Hashimoto, M Shiiba, S Katoh, ... Process Biochemistry 41 (3), 505-511, 2006 | 36 | 2006 |