Tetsushi Sakuma
Tetsushi Sakuma
Associate Professor (Lecturer), Hiroshima University
確認したメール アドレス: hiroshima-u.ac.jp - ホームページ
タイトル引用先
Precise correction of the dystrophin gene in duchenne muscular dystrophy patient induced pluripotent stem cells by TALEN and CRISPR-Cas9
HL Li, N Fujimoto, N Sasakawa, S Shirai, T Ohkame, T Sakuma, ...
Stem cell reports 4 (1), 143-154, 2015
3632015
Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9
S Nakade, T Tsubota, Y Sakane, S Kume, N Sakamoto, M Obara, ...
Nature communications 5, 5560, 2014
2292014
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
T Sakuma, A Nishikawa, S Kume, K Chayama, T Yamamoto
Scientific reports 4, 5400, 2014
1952014
Efficient TALEN construction and evaluation methods for human cell and animal applications
T Sakuma, S Hosoi, K Woltjen, K Suzuki, K Kashiwagi, H Wada, H Ochiai, ...
Genes to Cells 18 (4), 315-326, 2013
1682013
Robust in vitro induction of human germ cell fate from pluripotent stem cells
K Sasaki, S Yokobayashi, T Nakamura, I Okamoto, Y Yabuta, K Kurimoto, ...
Cell stem cell 17 (2), 178-194, 2015
1602015
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity
T Sakuma, H Ochiai, T Kaneko, T Mashimo, D Tokumasu, Y Sakane, ...
Scientific reports 3, 3379, 2013
1552013
Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases
T Watanabe, H Ochiai, T Sakuma, HW Horch, N Hamaguchi, T Nakamura, ...
Nature communications 3, 1017, 2012
1532012
Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice
T Aida, K Chiyo, T Usami, H Ishikubo, R Imahashi, Y Wada, KF Tanaka, ...
Genome biology 16 (1), 87, 2015
1512015
Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish
Y Hisano, T Sakuma, S Nakade, R Ohga, S Ota, H Okamoto, T Yamamoto, ...
Scientific reports 5, 8841, 2015
1362015
MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems
T Sakuma, S Nakade, Y Sakane, KIT Suzuki, T Yamamoto
Nature protocols 11 (1), 118, 2016
1262016
Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases
S Ansai, T Sakuma, T Yamamoto, H Ariga, N Uemura, R Takahashi, ...
Genetics 193 (3), 739-749, 2013
1062013
Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato
S Sawai, K Ohyama, S Yasumoto, H Seki, T Sakuma, T Yamamoto, ...
The Plant Cell 26 (9), 3763-3774, 2014
842014
Simple knockout by electroporation of engineered endonucleases into intact rat embryos
T Kaneko, T Sakuma, T Yamamoto, T Mashimo
Scientific reports 4, 6382, 2014
782014
EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step
S Ninagawa, T Okada, Y Sumitomo, Y Kamiya, K Kato, S Horimoto, ...
J Cell Biol 206 (3), 347-356, 2014
752014
Human induced pluripotent stem cell–derived podocytes mature into vascularized glomeruli upon experimental transplantation
S Sharmin, A Taguchi, Y Kaku, Y Yoshimura, T Ohmori, T Sakuma, ...
Journal of the American Society of Nephrology 27 (6), 1778-1791, 2016
722016
High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos
TS Ken-ichi, Y Isoyama, K Kashiwagi, T Sakuma, H Ochiai, N Sakamoto, ...
Biology open 2 (5), 448-452, 2013
712013
Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
T Mashimo, T Kaneko, T Sakuma, J Kobayashi, Y Kunihiro, B Voigt, ...
Scientific reports 3, 1253, 2013
652013
Generation of a nonhuman primate model of severe combined immunodeficiency using highly efficient genome editing
K Sato, R Oiwa, W Kumita, R Henry, T Sakuma, R Ito, R Nozu, T Inoue, ...
Cell Stem Cell 19 (1), 127-138, 2016
592016
The microtubule-depolymerizing activity of a mitotic kinesin protein KIF2A drives primary cilia disassembly coupled with cell proliferation
T Miyamoto, K Hosoba, H Ochiai, E Royba, H Izumi, T Sakuma, ...
Cell reports 10 (5), 664-673, 2015
582015
Nuclease‐mediated genome editing: At the front‐line of functional genomics technology
T Sakuma, K Woltjen
Development, growth & differentiation 56 (1), 2-13, 2014
582014
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論文 1–20